itgavb3  (R&D Systems)

Journal: Bioengineering & Translational Medicine

Article Title: High‐dose vitamin D metabolite delivery inhibits breast cancer metastasis

doi: 10.1002/btm2.10263

Figure Lengend Snippet: In vivo metallopeptidases and focal adhesion‐relative proteins detection. (a) The upregulation of MMP2 or MMP facilitate metastasis of breast cancer. (b) MMP2 and (c) MMP9 detections in tumors. (d) Main components illustration of focal adhesion. (e) Actin, (f) paxillin, and (g) integrin αvβ3 expression detections in tumors treated as indicated. *** p < .001

Article Snippet: Then, the whole cell lysis was detected by ELISA Kits (Mouse MMP‐2 Elisa Kit (RAB0366; Sigma‐Aldrich); Mouse pro‐MMP‐9 Elisa Kit (RAB0373, Sigma‐Aldrich); ACTB/Beta Actin Elisa Kit (LS‐F10737‐1, LSBio); PXN/Paxillin Elisa Kit (LS‐F21248‐1, LSBio); Mouse Integrin Alpha V Beta 3 (ITGAVB3) Elisa Kit (MBS9362139_48wells, MyBioSource)) according to corresponding instructions.

Techniques: In Vivo, Expressing

Journal: Endocrinology

Article Title: Autocrine and paracrine mechanisms of prostaglandin E₂ action on trophoblast/conceptus cells through the prostaglandin E₂ receptor (PTGER2) during implantation.

doi: 10.1210/en.2012-2271

Figure Lengend Snippet: Figure 5. PGE2 (100 nM) Induces Increased Adhesion of Primary Porcine Trophoblast Cells (A) and Human Trophoblast HTR-8/SVneo Cells (B) to ECM Fibronectin. Both types of cells were preincubated with either vehicle, PTGER2 antagonist (10 M AH609), 1 mM RGDS, or 1 mM RGES peptides, integrin (anti-ITGAVB3) antibody, or 50 g IgG for 30 minutes and then seeded onto fibronectin-coated plates in the presence of vehicle or 100 nM PGE2 for 1 hour (HTR-8/SVneo cells) or 3 hours (primary porcine trophoblast cells). Cell adhesion was calculated as n-fold increase relative to the control. Each treatment was carried out in triplicate, and each bar represents the mean SEM of at least 5 independent experiments. Bars with different letters (a–c) indicate significant differences (P .05).

Article Snippet: In main experiment, isolated porcine trophoblast cells and HTR-8/SVneo cells were incubated in phenol red-free medium 199 containing 1% NCS and RPMI containing 1% FBS, respectively, in the presence/absence of 1 mM arg-gly-asp-ser (RGDS; ITGAVB3-directed tetrapeptide arg-gly-asp-ser), 1 mM arg-glyglu-ser (RGES) tetrapeptide, 10 M AH6809, 50 g anti-ITGAVB3 antibody (integrin v 3; SC-7312, Santa Cruz Biotechnology, Inc. Santa Cruz, California) for 30 minutes prior to the addition of vehicle or 100 nM PGE2.

Techniques: Control